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Developed a novel C. elegans model of Alzheimer’s disease

· Conceptualized the design of novel C. elegans model to study the pathogenesis of Alzheimer’s disease.

· Completed analysis of data acquired from confocal microscopy of synaptic puncta in wildtype and transgenic worms and represented data in graphical formats.

· Maintained accurate records of research findings and provided statistical analysis of data results from lifespan assays of transgenic vs wildtype C. elegans.

· Collaborated with Associate Professor, Molecular Biosciences department to advance research and gain deeper understanding of the disease pathogenesis using C. elegans.

· Presented research results at symposium.

· Replenished stock levels, repaired broken equipment and enforced sterilization procedures.

Effects of mutant amyloid precursor protein (APP) on human tau protein

· Initiated and maintained cultures of neuroblastoma cell lines and HEK cell lines in a sterile environment.

· Performed transfection of neuroblastoma cells to obtain APP mutant cell lines.

· Troubleshot and developed experimental tools to test the effects of specific APP mutants on the expression and phosphorylation of tau protein by qRT-PCR and western blotting using phosphorylation antibodies.

· Collaborated with colleagues to conduct research in neuroblastoma cell lines and gather results.

· Leveraged interpersonal and communication skills to mentor 3 PhD and graduate students.

Identified RNAi reagents that have improved effectiveness in eliciting C. elegans male progeny.

· Formulated an RNAi methodology that knocked down two C. elegans genes simultaneously to result in Him phenotype.

· Collected, digitally documented and critically analyzed data that led to a publication.

· Wrote and reviewed standard operating procedures in the laboratory.

Designed and executed molecular genetics experiments towards characterizing functional protein partners of ABC transporters.

· Constructed recombinant transgenic reporters using “Split-GFP Reconstitution” system to detect binding protein partners.

· Optimized PCR and recombinant DNA expression of 12 C. elegans ABC transporter genes.

· Verified success of cloning by Sanger sequencing.

· Troubleshot problems with equipments and experiments and obtained intended results.

· Trained five graduate students and fourteen undergraduate students technically and provided guidance with research projects.

· Effectively communicated scientific data through write-ups, posters and in-lab and departmental presentations.

Demonstrated a novel nrde-3 dependent nuclear silencing in an endogenous locus in C. elegans.

· Performed qRT-PCR experiments to determine trans-splice mRNA levels of eri-6/7 locus.

· Identified a candidate gene that might have a role in transgenic silencing by sequencing whole genome of a C. elegans strain via Next Generation Sequencing.

· Managed laboratory responsibilities including but not limited to calibration of instruments, safe disposal of hazardous wastes, keeping up with lab stock requirements and ordering supplies.

Investigated the mechanism of resistance against silver nanoparticles (SNP) in bacterial population.

• Studied interaction of SNPs with extracellular protein (ECP) produced by two environmental bacteria and the adsorption behavior in aqueous solutions.
• Conducted adsorption isotherm and kinetics experiments and collected data using spectrophotometry and Dynamic Light Scattering.

Tested the use of natural dyes to stain biological molecules like protein and DNA.

• Pursued molecular biology methods to extract natural dye from Bixa orellana seeds and researched its application as an electrophoretic tracking dye.